Biocompatibility of annulus fibrosus-derived stem cells with porcine decellularized annulus fibrosus matrix

doi:10.3969/j.issn.2095-4344.2014.41.017

Abstract

Abstract:

BACKGROUND: Acellular matrix and annulus fibrosus-derived stem cells are both derived from the annulus tissue, and their tissue engineering complexes may have better biocompatibility.

OBJECTIVE: To culture rabbit annulus fibrosus-derived stem cells on the porcine decellularized annulus fibrosus matrix, and to observe the growth of annulus fibrosus-derived stem cells on the decellularized matrix scaffold.

METHODS: Decellularized annulus fibrosus matrix from porcine was prepared and detected by scanning electron microscopy, DAPI staining and Fourier transform infrared spectroscopy analysis. After isolation and culture, annulus fibrosus-derived stem cells were seeded onto the decellularized annulus fibrosus matrix. Cell growth on the scaffolds was observed by cytoskeleton staining, inverted immunofluorescence microscopy and scanning electron microscopy to draw cell growth curve.

RESULTS AND CONCLUSION: The prepared decellularized annulus fibrosus matrix was white and translucence liquid. The result of Fourier transform infrared spectroscopy indicated that the main component of the decellularized annulus fibrosus matrix was collagen. DAPI staining and scanning electron microscopy results showed no cells resident. The cytoskeleton staining displayed that annulus fibrosus-derived stem cells grew well on the scaffolds at 1, 3, 7 days. These findings indicated that annulus fibrosus-derived stem cells have a good biocompatibility with the decellularized annulus fibrosus matrix in vitro.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, fibrocartilage, extracellular matrix, materials testing, biocompatible materials

CLC Number:

R394.2

Liu Chen, Wang Sheng-hao, Wang Yi-bin, Tang Xue-bin, Yang Hui-lin, Li Bin. Biocompatibility of annulus fibrosus-derived stem cells with porcine decellularized annulus fibrosus matrix[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(41): 6655-6660.

Figures/Tables 2

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